ca xii sirna Search Results


ca xii sirna  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology ca xii sirna
Effects of A6 and A15 on the viability of MCF-7 and SK-BR-3 cells transfected with <t>siRNA</t> targeting CA IX, II, or <t>XII.</t> MCF-7 and SK-BR-3 cells were transfected with control siRNA or siRNA targeting ( A ) CA IX, ( B ) CA II, and ( C ) CA XII. Western blotting analyses were performed as described in the Materials and Methods to confirm the efficiency of the respective knockdown. Representative blots are shown. Transfected cells were exposed to A6 or A15 at 50 µM for 48 h. Control cells were treated with the media containing 0.1% DMSO instead. Cell viability was determined by MTT assay as described in the Materials and Methods and expressed as a percentage of the control measured in the vehicle (DMSO)-treated cells transfected with control siRNA. Data are displayed as mean ± SEM from at least three independent experiments. * p < 0.05 vs. the vehicle-treated control cells transfected with control siRNA. ns, not significant.
Ca Xii Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of A6 and A15 on the viability of MCF-7 and SK-BR-3 cells transfected with siRNA targeting CA IX, II, or XII. MCF-7 and SK-BR-3 cells were transfected with control siRNA or siRNA targeting ( A ) CA IX, ( B ) CA II, and ( C ) CA XII. Western blotting analyses were performed as described in the Materials and Methods to confirm the efficiency of the respective knockdown. Representative blots are shown. Transfected cells were exposed to A6 or A15 at 50 µM for 48 h. Control cells were treated with the media containing 0.1% DMSO instead. Cell viability was determined by MTT assay as described in the Materials and Methods and expressed as a percentage of the control measured in the vehicle (DMSO)-treated cells transfected with control siRNA. Data are displayed as mean ± SEM from at least three independent experiments. * p < 0.05 vs. the vehicle-treated control cells transfected with control siRNA. ns, not significant.

Journal: International Journal of Molecular Sciences

Article Title: Suppression of Tumor Growth and Cell Migration by Indole-Based Benzenesulfonamides and Their Synergistic Effects in Combination with Doxorubicin

doi: 10.3390/ijms23179903

Figure Lengend Snippet: Effects of A6 and A15 on the viability of MCF-7 and SK-BR-3 cells transfected with siRNA targeting CA IX, II, or XII. MCF-7 and SK-BR-3 cells were transfected with control siRNA or siRNA targeting ( A ) CA IX, ( B ) CA II, and ( C ) CA XII. Western blotting analyses were performed as described in the Materials and Methods to confirm the efficiency of the respective knockdown. Representative blots are shown. Transfected cells were exposed to A6 or A15 at 50 µM for 48 h. Control cells were treated with the media containing 0.1% DMSO instead. Cell viability was determined by MTT assay as described in the Materials and Methods and expressed as a percentage of the control measured in the vehicle (DMSO)-treated cells transfected with control siRNA. Data are displayed as mean ± SEM from at least three independent experiments. * p < 0.05 vs. the vehicle-treated control cells transfected with control siRNA. ns, not significant.

Article Snippet: CA II, CA IX, and CA XII siRNA and control siRNA were provided by Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

Techniques: Transfection, Control, Western Blot, Knockdown, MTT Assay